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Sequential files are generally stored in some sorted order (e.g., alphabetic) for printing of reports (e.g., a telephone directory) and for efficient processing of batches of transactions.
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Even if some sorted cells were, in fact, aggregates, their frequency in the FCM-sorted cell group must have been very rare (less than 0.44% or 1/228).
Hence, the process can be continued in the same fashion, outputting the hyperpaths in H in sorted order.
In some experiments, the sorted cells were seeded within growth factor-reduced Matrigel and cultured in the presence of Human or Mouse EpiCult-B and irradiated feeders for 14 to 21 days.
In some wells, FACS-sorted CD4+6.5+CD25− or CD25+ T cells obtained from dtg mice were added to CD4+6.5+CD25− from stg mice at a ratio of 1∶1.
In some experiments, negatively-sorted DNCD3 splenic cells on mouse CD4/CD8 tandem columns cells were cultured for 5 days in Th1 conditioned medium (10% FCS RPMI medium supplemented with 10 U/ml rIL-12, 10 µg/ml anti-IL-4 mAb, and 2.5 µg/ml CD3/CD28 Abs) or in Th2 conditioned medium (10 µg/ml anti-IFN-γ mAbs and 2.5 µg/ml CD3/CD28 Abs).
Therefore, some rare templates may be amplified in sorted samples and not in filtered samples, because template competition is lower in the former samples.
Without telling anyone, Kiefer began collecting the items, stashing them in sorted piles in the garages of friends.
The trend in the conductance in unsorted and sorted samples is confirmed by current-voltage measurements.
Conversely, a list of genes detected in whole tissue but not in sorted cells was also compiled.
Cell cycle phase distribution was determined by standard Propidium Iodide staining in control bone marrow cells and in sorted, RFP-negative cells (no Mybl2 RNAi) and in sorted, RFP-positive cells (Mybl2 knockdown).
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