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It is evident that chromatin is aggregated in some nucleus regions, while other areas lack the presence of chromatin.
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Immunohistochemical analysis of Rb distribution did not reveal any obvious alteration besides a decreased Rb signal in some nuclei of cells from subjects with HGPS (Figure S7).
TAMs had brown CD68 staining mainly in the cytoplasm, but also in some nuclei.
Staining decreased at 6 months in WT thyroid, but was unchanged in RET/PTC3 follicular cells (visible in some nuclei).
In some nuclei, several other weakly CMA3+ regions not corresponding to DAPI- signals mostly with pericentromeric locations were observed.
In postnatal stages, including the adult, some fibre cell nuclei appeared less PROX1-immunoreactive (not shown), and the fluorescence was barely detectable in some nuclei.
MMP-15 showed a strong staining in the cytoplasm of tumor cells with additional slightly staining of few stromal cells and in some nuclei of tumor cells.
d-Mannose very weakly triggered the nuclear localization; strong GFP fluorescence was observed only in some nuclei at the concentration of 3 mM.
We observed two distinct GFP dots representing unpaired homologs in some nuclei in TZ, and one GFP dot (or two closely associated dots) in basically all nuclei in the middle region of a gonad that corresponds to the pachytene stage, which represent paired homologous loci.
When compared to p27, the cyclin D1 is expressed in some nuclei of luteal cells only (arrow, panel C) and absent from the stromal cells in the CL of pregnancy.
However, we did observe HipHop foci in some nuclei, suggesting that the effect of loss of ATM on HipHop loading is partial, similar to the situation in larval neuroblasts.
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