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The seasonal behavior of water level in SGP strongly indicates that monsoon rainfall plays an important role in shallow aquifer recharge.
To find novel genes involved in SGP development, we used a weak allele of ehn-3 as the basis for a reverse genetic screen.
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We asked if SWI/SNF mutants also express arg-1 :: GFP in SGPs.
Shibata et al. (2012) reported that pbrm-1 mutants have reduced hnd-1 :: GFP expression in SGPs.
In wild-type larvae, arg-1 :: GFP is expressed only rarely and at very low levels in SGPs.
We used the hnd-1 promoter, which is expressed broadly in the MS, C, and D lineages and later in SGPs (Mathies et al. 2003).
The hunchback and Ikaros-like gene ehn-3 is expressed specifically in SGPs and is required for their development into differentiated tissues of the somatic gonad.
Similarly, we hypothesized that EHN-3 might establish a permissive chromatin state in SGPs via direct interactions with chromatin remodeling complexes such as SWI/SNF.
Despite the importance of ehn-3 for gonadogenesis, it is not known how this zinc finger transcription factor acts in SGPs to control their subsequent development.
To ascertain if Vasa is required in SGPs, their behaviour in vasa zygotic null embryos was examined using antibodies against AbdB and against the general SGP marker Eyes absent (Eya) also known as Clift (Boyle et al., 1997).
The C. elegans HIL gene family includes ehn-3, which is expressed specifically in SGPs and is important for the development of several differentiated tissues of the somatic gonad (Large and Mathies 2010).
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