Milligram quantities of GPCRs can now be expressed in several expression systems and purified to high homogeneity.
However, a synthetic version of MVP (MVP-s), in which 62 of the archaeal codons were altered to bacterial codons (see Experimental Procedures), was successfully expressed in several expression vectors and cell lines (Table 1).
We decided to use clec-67 as a marker of C. elegans immunity to S. enterica because it is consistently upregulated by this pathogen in several expression profiling experiments performed in our laboratory, because it is expressed in the intestine (Figure 2A), and because its promoter region contains a GATA binding site (A/T GATA A/G).
To test if ELF6 and AtJmj4 are active histone demethylases, we tried to express the full-length ELF6 and AtJmj4 proteins in several expression systems.
Since one of the goals of our lab is to express recombinant proteins (using plant viral vectors), we often need to clone specific genes of interest in several expression vectors [14].
These genes were observed to be upregulated upon exposure to pathogens in several expression profiling studies [ 14, 15] suggesting a role in immune response.
In fact, several expressions commonly understood in the West including one for fear have very different meanings to one indigenous, isolated society in Papua New Guinea.
CCMV VLPs have been expressed in several heterologous expression systems i.e. yeast [ 24], insect cell (unpublished observation), prokaryotic [ 19] and analyzed for VLP formation.
In addition to our RA treatment analysis, knockdown analyses of actinotrichia proteins, which are components of fin rays and lost in tetrapod, show an anterior shift in several gene expressions in zebrafish pectoral fin buds (Zhang et al., 2010).
To facilitate these studies, expression of the HIV-1 gp120 envelope glycoprotein has been done in several over-expression settings.
