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The reasons for a large number of wheat/barley unigenes without matches to rice genomic sequence include not having the complete sequence of the rice genome available (the majority of clones were still in sequencing phase 2, with gaps) and having a relatively high stringency setting for filtering wheat and barley sequence comparisons to rice.
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Moreover, the positive effect of better assignment and classification of long reads comes with higher costs and workload in the sequencing phase.
In the sequencing phase of the Arabidopsis Genome project, it was agreed that each group would continue sequencing up to the region containing intractable centromeric repeats.
In the initial random sequencing phase, approximately 6-fold sequence coverage was achieved from the two libraries (each library was sequenced to 3-fold coverage, respectively).
The barcoding strategy is similar to RAD but modulation of barcode nucleotide composition and length results in fewer sequence phasing errors.
The synovial membrane thickness (mm) was measured with the In-Out Phase sequence and measured in four subregions (medial and lateral articular recess and medial and lateral border of the suprapatellar bursa) as described [ 17, 18].
In sequence, we compute phase-transition pressures, the second-order aggregate elastic constants within the Shell model.
The exact palindromic motif calculated for a particular ORF, or from datasets representing multiple ORFs, varied in sequence, length and phasing, relative to the repeating protein domain.
SEEDS course comprises of two different steps in sequence: an initial learning phase and a project work phase.
Axial T1-weighted gradient echo sequence, in-phase and opposed-phase (TR 180 ms, TE 2.3 ms/4.6 ms, flip angle 90°, breath-hold), preferably as a dual-echo sequence.
We found that a mixture of (Ti,V Al3 and (Ti,V Al2 phases exist in the outer layer and that (Ti,V Al2, (Ti,V Al, and (Ti,V 3Al phases form in sequence within the reaction zone after oxidizing for up to 96 h.
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