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The AT1 expression pattern identified with the primary anti-AT1 antibody from Santa Cruz was identical to that detected in select sections with an alternative anti-AT1 antibody purchased from Sigma (data not shown).
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Adjacent normal kidney served as an internal positive control in selected sections.
Anti-CD68 (PG-M1) (Dako, Denmark) was used in selected sections to identify macrophages in mononuclear cell populations.
By contrast, IHC analysis morphologically distinguishes tumor cells from normal cells and specifically evaluates staining intensity and amount of tumor cells in selected sections.
Supplementary Figure 2 shows the precise location of injection sites and the pattern of projections in selected section contours for 28 target areas (all but F2).
The severity of the axonal ataxin-3 pathology was assessed in select tissue sections.
DCX expression was quantified by measuring optical density with a dedicated stereological computer program [49], for one in every six coronal sections of the OB and in equivalent selected sections containing the SVZ.
In addition, selected sections were stained with elastic Van Gieson (EVG) and Masson trichrome in order to evaluate the extra-cellular elastic and collagen fibers, respectively.
The HRSG is modeled in 1/10 scale-down and velocities are measured by an anemometer device (KM 909 model) at diverse points in its selected sections.
Third, a separate list of boys in the selected sections was developed by unit leaders of the sections and then eligible boys were recruited from the list of male students using simple random sampling method.
For comparison, in some selected sections we used the ChAT antibody (ChAT rabbit polyclonal; Advanced Targeting Systems, San Diego, CA, USA), which produced the same staining pattern and the same proportion of cells as that obtained with AB143 (Millipore).
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