Sentence examples for in same buffer from inspiring English sources

The active fractions were collected, added to ammonium sulfate at 30% saturation, and subjected to a Butyl-TOYOPEARL® 650 M column equilibrated with 30% saturation of ammonium sulfate in same buffer.

The beads were stored in same buffer.

ATP or MgATP were prepared in same buffer before addition to protein samples.

The gut extract was incubated at room temperature on a rotator shaker with 0.5 mg/ml Micrococcus lysodeikticus cells prepared in same buffer for 24 h.

After rinsing in same buffer the tissue was dehydrated through a series of graded ethanol to propylene oxide, infiltrated and embedded in epoxy resin and polymerized at 70°C overnight.

Briefly, 5×105 M17 cells or cell lines expressing PrPC and mutant PrP forms plated in 35 mm Petri dishes were washed with PBS containing 1 mg/ml BSA and 20 mM Hepes, pH 7.3 and incubated with 0.25 µM calcein-AM for 20 min at 37°C in same buffer.

The experiments performed under non-reducing conditions were performed in the same buffer, in the absence of DTT.

Electrophoresis was carried out in the same buffer in the dark for 30 min at 25 V and 300 mA.

Cells were then washed in the same buffer, dehydrated in acetone, and embedded in PolyBed 812 (Polyscience, Philadelphia, PA, USA).

The cells were then washed in the same buffer, dehydrated in acetone, and embedded in Epon.

Parasites were washed in the same buffer, dehydrated in acetone and embedded in Epon resin.