Sentence examples for in run buffer from inspiring English sources

After photopatterning, the fsPAG was soaked in run buffer for 10 min on an Orbitron shaker.

PCR product was hybridized to fresh Firefly Multimix for 30 minutes at 37°C and re-suspended in Run Buffer for readout.

The column was equilibrated in running buffer (2 × PBS (phosphate-buffered saline)) and calibrated with different standard proteins: Tyroglobulin (660 kDa), Ferritin (440 kDa), Catalase (240 kDa), and BSA (bovine serum albumin) (67 kDa).

Low melting point agarose (0.5% in running buffer containing BPB) was layered on top of the strip.

A Superdex 200 column was equilibrated in running buffer and 200 μl of the sample was run through the column at a rate of 0.5 ml min−1.

A Superdex 75 column was equilibrated in running buffer and 100 μl samples were run through the column at a rate of 0.5 ml min−1.

Purified fusion protein was diluted to 2 mg ml−1 in running buffer (20 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 8).

Different concentrations of peptides (33 nM–2.7 μM in running buffer) were applied to the chip surface at a flow rate of 10 μl min−1.

In the enhanced format the assay had limits of detection (LOD) of 3.7 pg ml−1 with standard in running buffer, and 15.4 pg ml−1 in a stripped human saliva matrix.

Cell lysates were heated to 95 100 °C for 5 min in sample buffer (187.5 mM Tris-HCl, 6% w/v SDS, 30% glycerol, 150 mM DTT, 0.03% w/v bromphenol blue), and an equal amount of protein was loaded on precast SDS-PAGE (Thermo Fisher Scientific) in running buffer and transferred to nitrocellulose membrane.

Twenty-four hours after transfection, the cell lysate was fractionated by 8% Native Gel in running buffer containing 1% sodium deoxycholate at 4°C, as previously described (Frieman et al., 2009).