Primary symptoms include extremely poor reading skills owing to no apparent cause, a tendency to read and write words and letters in reversed sequences, similar reversals of words and letters in the person's speech, and illegible handwriting.
The cross-over designed study provided to all participants both types of interventions, the motivational as well as the self-regulatory intervention, either in the theory-based or in the reversed sequence (Mot-SelfR versus SelfR-Mot).
This association has been previously observed in a reversed sequence: increased risk of melanoma after squamous cell carcinoma (Hemminki and Dong, 2000).
Peptide spectra with a Mascot score of 20 or greater and with no ambiguous hits in a decoy (reversed sequence) database were manually inspected, and the verified peptides were selected as the HDX peptide set.
Under such criteria, all MS/MS identifications of peptides present in entries with reversed sequences (i.e., false-positive identifications) were not validated, since none of them were identified with 2 peptides with score higher than 21 each or 1 peptide with score higher than 30.
False discovery rates (FDR) were estimated based on matches to reversed sequences in the concatenated target-decoy database, and an FDR threshold of 1% was used for proteins and peptides.
Paragon searches were conducted in "Thorough Mode" using a reversed sequence database to obtain 95%% peptide identification confidence.
The reversed sequence (first shock, then sucrose) was employed in another group of bees tested in parallel (n = 104).
In contrast to the widespread discretization sequence, first space then time, the reversed sequence first time then space is employed.
The raw files were processed with MaxQuant (version 1.2.2.5) [ 89] and searched against the B. distachyon protein database (31,029 entries in total) in Phytozome (http://www.phytozome.net/search.php; version 9.1) concatenated with a decoy of reversed sequences.
This obvious reversed sequence of the opening/closing of valves and squeezing motion of the Vegfc2195 heart also remains in larval stage (Movie S4).
