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The phaC Bs was also cloned in reverse orientation to lac promoter in pBBR1MCS-2 to confirm the expression under its native promoter.
To examine whether the expression of phaC Bs was affected by the lac promoter, pBBR1MCS-2 phaC Bs BE was constructed by inserting phaC Bs in reverse orientation to lac promoter.
The rearrangement suffered by the T-DNA during the integration process placed a truncated 35S promoter in reverse orientation to the tagged gene.
For the construction of down-regulation vectors, a cDNA fragment of the target gene should be inserted into both MCS sites, in reverse orientation, to form inverted repeats (IR), hairpin RNA structures that can specifically silence gene expression via the mechanism of RNAi.
Lists of those probesets detected, differentially expressed, or annotating in reverse orientation to RefSeq sequences were created and used by the script to further subdivide the 9 categories.
The plasmid pRed-Caps-Lib was created incorporating the AAV8 rep/cap gene expressed from a PGK promoter in reverse orientation to the dsRed gene expressed from a CMV promoter.
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For between copy primers the sequences were cut in half and pasted in a reverse orientation to generate properly directed primer sets.
Some templates produced a double sequence close to the P0 site, these were sequenced using another primer that binds in the reverse orientation to a conserved region in the middle of the 16S region (095P: 5'-TAC GGC GTG GAC TAC CAG-3').
One of the binding sites occurs in a reverse orientation to the other.
The human AIM2 gene, like mouse Aim2, lies in the reverse orientation to other genes at one end of the gene cluster near the CADM3 gene.
Cluster1 (OBP22 27) is located on chromosome 5. OBP26 is present in the reverse orientation to the other members of the cluster.
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