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It is flanked by perfect direct repeats (one orientated in reverse orientation) and is integrated between two asn tRNAs, of which one in the reverse orientation.
However, this region is flanked by perfect direct repeats (one orientated in reverse orientation), and it is integrated between two asn tRNAs, of which one is in the reverse orientation.
However, we orient BAC PGEC472P22 (accession AC151815) of A in reverse orientation compared to [ 25], thereby introducing the inversion of A and R1 relative to r1, B and C (see Discussion).
The phaC Bs was also cloned in reverse orientation to lac promoter in pBBR1MCS-2 to confirm the expression under its native promoter.
Fig. 1 Characterization and phenotypic analysis of the mutants (KO). a OsINV3 gene in 5′-3' direction with the Tos17 insertion in the second exon in reverse orientation.
A short fragment of lacZ was cloned into the pBAD expression vector, in reverse orientation, using the EcoRI and PstI restriction sites.
To examine whether the expression of phaC Bs was affected by the lac promoter, pBBR1MCS-2 phaC Bs BE was constructed by inserting phaC Bs in reverse orientation to lac promoter.
The Ter sequence has been presented in reverse orientation [11].
It codes for the essential D4R gene in reverse orientation.
As controls we transfected empty vectors or vectors with the 5'UTRs in reverse orientation.
Again, as a control, dfoxO IRES translation was inactive with the 5'UTR inserted in reverse orientation (Fig. 3B).
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