In response we developed a novel experimental model using lethal toxin (LeTx), an anthrax toxin-derived pan-MKK protease, and genetically engineered protease resistant MKK mutants (MKKcr) to test the sufficiency of MEK signaling in melanoma SK-MEL-28 cells.
To better understand the molecular and cellular basis of this response, we have engineered an in vitro model of acetone-induced injury using organotypic epidermal cultures.
To evaluate the role of individual PTDs in induction of immune responses in tumor vaccination studies, we engineered recombinant fusion Ovalbumin (OVA) that contained three differrent PTDs, including the most efficacious known PTD (polyarginine (R9 -PTD).
In this study, we engineered a Lactococcus (L).
In this study, we engineered isogenic cell line pairs with and without p53 function using RNA interference (RNAi) and examined the stress responses of parent and RNAi-transduced cell lines.
As shown in Figure 1A, we engineered three donor cassettes, one for each reading frame.
This affords the opportunity to probe both the local cellular environment and the protein distributions within cells in response to engineered surfaces.
To demonstrate that the response of the 14 new targets was due to base pairing with Qrr4, we engineered mutations disrupting the putative pairing regions in Qrr4.
An ability to predict or contain long-term fitness recovery in response to an engineered modification would benefit many applications in which the intent is to release the engineered organism as a free-living population.
"For example, you could post a video and ask students to answer a question about it, or post an article and ask them to write a paragraph in response," Google engineer Will Phan explains.
For this purpose, we engineered an in situ rescue system.
