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Furthermore, endothelial marker, VE-cadherin, was downregulated and mesenchyme markers, N-cadherin and Snail, were induced in response to expression of a constitutively active form of Akt1 (myrAkt1) in endothelial cells.
In vivo cortical migration was disrupted more dramatically in response to expression of dominant negative dynamin compared to dominant negative clathrin.
To further test the potential role of JNK1 and JNK2 in mtUPR signaling, we determined the effect of the MEK inhibitor PD98059 [22], [22] on JNK phosphorylation in response to expression of OTCΔ.
However, HEK-293 cells, which showed inhibition of mTOR signaling in response to expression of CD44-CP1 (Figure 2C and 2D), were non-ciliated (≤2%), suggesting that repression of mTOR signaling was a cilia-independent function of CP1.
To determine whether the AR was functionally active, we analyzed hypothalamic samples for AR activation in response to expression of p21, which regulates cell-cycle progression and correlates inversely with the levels of active AR.
Expression of Casp8FL resulted in an increase in p24 production relative to control plasmid, yet this effect was significantly less than the amount of p24 produced in response to expression of Casp8p41 (Figure 7).
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Our analysis revealed that over 38 genes were changed in their expression in response to TS expression (n = 3, p < 0.05 and 4-fold cut-off).
Dual vector administration failed to provoke antibody formation in response to GAA expression with AAV-CBhGAA; however, hepatic-restricted expression from dual vector expression did not prevent antibody formation after a strong immune challenge with GAA and adjuvant.
Its expression in the A compartment increased in response to ectopic expression of Hh but was insensitive to Dpp.
Pathway activity is triggered in response to endogenous expression of Hh ligands, and is dependent upon the expression of Smoothened, an essential Hh response component that is not expressed in benign prostate epithelial cells.
However, expression of Atg6/Beclin1 was not significantly changed in response to Rab25 expression (Fig S3B of Supporting information).
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