(Similar differences in translational repression between cap and cap&tail forms of target mRNA were seen in undifferentiated P19 cells co-transfected with synthetic let-7 (Fig. S4)).
A significant difference in repression strength scores was again found between evolutionary paths were GAL80 duplicate loss is the last event and all other paths (-0.40 +/- 0.23 versus -2.08 +/- 1.03, P < 0.01, Additional File 1, Figure S4c).
Importantly, the final position of the boundaries of the p3 and pMN progenitor domains is regulated, at least in part, by cross repression between these factors (Briscoe et al., 1999, 2000; Ericson et al., 1997; Novitch et al., 2001).
Correspondingly, our knock-down analysis of the gap gene network in M. abdita reveals a stronger posterior-to-anterior bias in cross-repression between neighbouring domains.
One of the earliest patterning events is the mutual transcriptional repression between Gli3 in the anterior tissue and Hand2 in the posterior (Te Welscher et al., 2002; Osterwalder et al., 2014).
Recapitulating the dynamic behavior of the circuit in silico only requires mutual repression between the transcriptional networks underlying the Epi and the PrE fates without any positive feedback loops, and a single repressive input of MAPK signaling on the Epi-specific program.
"I am more interested in repression than depression".
But the commander nevertheless still puts his trust in repression.
We observed a good correlation between gene repression and the repressive mark H3K27 trimethylation (Figures 5D, 6D and 7D), suggesting that EBNA3B, as well as EBNAs 3A and 3C, is involved in repression through polycomb recruitment.
This feature of "overshoot" in temporal gene expression between the Delay and the Basic circuit is, therefore, primarily due to the delay in repression.
