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Each patient's RNA sample (PT1, PT2, PT3, PT4) was co-hybridized with a control's pooled RNA (C1+C2+C3) and analyzed in replicate with dye swap, for a total of 8 slides (n = 8).
CHART-seq was performed in replicate with two independent pull down samples and matched controls using non-targeting LacZ oligos.
At each time point, thresholds at each location were determined in replicate, with one-minute interval between each measurement.
To examine the effect of serum depletion on CHO cell growth, cells were seeded in parallel NPD and DI based cultures in replicate with either 5% or 1% FCS.
The EBV BART1, BART2, and BHRF1 3 primers targeting EBV pre-miRNAs were applied in replicate with four replicate amplifications per primer pair, for a total of 12 results in each of 48 specimens, or 576 test results.
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The media in each well were removed, and the cells were treated and co-cultured in replicates with conjugated nano composite suspension (Au-CSCaCO3NP) for a period of 24, 48, and 72 h.
The RT-PCR was performed in replicates with 1 μl cDNA using the SYBR Green Master Mix Applied Biosystemss) with an ABI 7500 sequence detection system as prescribed in the manufacturer's protocol (Applied Biosystems).
Initially, eQTL were identified in the first replicate with a p-value cut-off of 1E-6 (Additional File 1) but were validated in replicate 2 with a less stringent cut-off of 1E-5 [ 21].
The distribution of the organisms in the culture-/PCR+ group was similar to the culture+/PCR+ group and was reproducible in replicate testing with an independent blood draw.
Gene-based analyses changed minimally with the two criteria sets (< 4% increase in the number of genes expressed in each replicate with an average of 1.6%).
These values are not significantly different from the with-in replicates variability.
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