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The full 70-mer complement was printed in replicate of four spots on the surface of a microarray slide.
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Nominal concentrations of 6, 20, 60, 200 and 600 mg PSOA/L were prepared in replicates of five, in addition to a control.
Serially diluted ATP standard solutions were used and 15 measurements in replicates of four for each ATP concentration (n = 60) were carried out.
All treatments were done in replicates of 6 wells.
Assays were performed in replicates of two or three on samples obtained from independent groups of mice.
Cells in each assay condition were incubated at 150,000 to 300,000 cells per well in 96-well microtiter plates, in replicates of 10.
LNCaP cells were plated in 96 well plates (5000 cells/well) in replicates of 6 wells per assay condition or incubation day.
All constructs were transfected in replicates of six wells within each assay, and each transfection assay was repeated a minimum of 3 times.
Two identical sub-arrays were printed per slide with each sub-array comprising 352 spots (80 glycans and 8 controls in replicates of 4).
Treatments were performed in replicates of six and the means expressed as the percent viability relative to the untreated control (100% viable).
This is consistent with the detection of significantly more FMDV genome copies/108 copies of 28 s rRNA in replicates of six GCs from mandibular lymph nodes, compared to similar replicates harvested from other tissues.
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