The combination of these two results led us to conclude that 5-hmC is not present in biologically relevant quantities in Arabidopsis DNA.
In order to achieve any impact on gene expression in a consuming organism, sRNAs would need to be absorbed and distributed in biologically relevant quantities within the cells of animal tissues and organs.
Although the generation of different mature hematopoietic cell types from PSC has been proven successful using classical two-dimensional (2D) differentiation cultures4,10,11,12,13, these systems do not allow for the generation of iPSC-derived cells in clinically relevant quantities.
"As long as you are working on your mouse or in vitro models," writes stem cell consultant Reinout Hesselink, "that is no problem". But this approach is not feasible to prep cells in clinically relevant quantities (millions and billions) that are necessary for patients when the time comes.
A gene transfer vector should be safe, stable, cost-effective to manufacture in clinically relevant quantities, and capable of efficient and tissue-specific delivery.
Taken together, our results led us to conclude that 5-hmC is not present in biologically relevant quantities within plant genomic DNA.
The great diversity of the B-lymphocyte repertoire implies that their interaction with the antigen induces a clonal expansion process (positive selection) resulting in the amplification of the number of clones and the production of specific antibodies against the pathogen or the vaccine in biologically relevant quantities.
For the plasma conditions considered, the magnitudes and spatial variations of the populations are a sensitive indicator of the accuracy of the radiation transport model, and are critical in calculating experimentally relevant quantities such as radiative powers and line ratios.
The present manuscript provides such an analysis by showing a way in which biologically relevant quantities, and their confidence intervals, can be rigorously computed from the primary data by means of kinetic models.
