For all other elements, we assume the TRLs to range from 'technology demonstrated in relevant environment (TRL 6)' to 'actual system proven in operational environment (TRL 9)' because of heritage from LPF and other missions.
The additional optical instruments and the external platform will reach the Technological Readiness Level (TRL) 'technology validated in relevant environment (TRL 5)' at the start of the BCD phases.
From these examples, it is clear that microfluidic in vivo mimetic systems enable evaluation of cellular processes in physiologically relevant environments, and the findings from such studies give critical insight into fundamental biological processes.
Hereby, solid materials can be immersed in chemically relevant environments, while the number of atoms traversed by the electron beam is reduced sufficiently to enable observations in situ by means of TEM.
These complex models would further our understanding of tissue compatibility and performance of the pattern in clinically relevant environments, before conducting clinical trials that would evaluate patient driven outcomes.
In particular, we could confirm that the observed anti-correlation between mRNA levels and aggregations propensities [30], [51], [52] is effectively translated to the protein level in physiologically relevant environments.
This paper describes an in vitro, 3D system based on soft lithography [5] that enables the culture of cells in biologically relevant environments at tissue-like cell densities (107 – 107 cells/cm3).
Developments in ecological momentary assessment (EMA) suggest the benefits of measuring symptoms at-the-moment in ecologically relevant environments [ 15].
Following, it is critically important to choose suitable cell and animal models to study these processes in physiologically relevant environments.
