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Here we employed a fast, high-efficiency labeling and inexpensive method based on dimethyl labeling, which has been used widely in relative quantitative proteomics.
Dimethyl labeling has been widely used in relative quantitative proteomics and it is fast, simple, reliable, cost-effective, and applicable to any protein sample, making it an ideal candidate method for the preparation of stable isotope-labeled internal standards.
In relative quantitative RT-PCR, the amount of target normalized to reference was calculated by: 2−ΔΔCt.
In relative quantitative RT-PCR, the β-actin gene was used as a reference gene, and the primers were 5'-AGATCGTGCGGGACATCAAG-3' (forward) and 5'-GCGGCAGTGGCCATCTC-3' (reverse).
In the latter, differences in gene expression were usually estimated as variation in relative quantitative expression, whereas in vertebrate systems only differences in spatiotemporal expression (i.e. anatomic domains at certain developmental stages) are assessed.
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For instance, since the deviations are used to assess the degree of selection at different codon positions, having a value to these deviations can help in understanding the relative quantitative differences in the strengths of selection at the three codon-positions.
In the ELK1 knock-down experiments, the relative quantitative analysis in binding activity was performed, utilizing densitometry and statistical analysis, by comparison of siELK1 with non-specific siRNA control, and the control was set at 1. ZW and PAB designed the experiment.
We found that after exposure to VEGF, mRNA and protein levels of LDLr were both increased significantly in PSFs, assessed using relative quantitative real-time RT-PCR and Western blot.
The study of dilution linearity and especially parallelism in performance verification of relative quantitative assays such as LBA cannot be over emphasised.
Such an approach has even greater relevance in all five categories of biomarker assays and especially in situations where QCs are usually less representative of clinical samples, such as in the case of relative quantitative techniques (Findlay, 2009).
The gene expression levels of ZO-1, CXCL16, CXCR6, IL-10, IFN-γ, TNF-α, Cx43, MUC2, and RegIIIγ in duodenal biopsies were assessed by relative quantitative reverse transcription-PCR in the HC and CD children.
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