Results were expressed in relative expression units.
Most of the inconsistent transcripts exhibit only minor changes in relative expression levels (~10%to20%0% increase or decrease) and relatively insignificant p values.
At least two-fold differences in relative expression, with a significance of p<0.05, were considered differentially expressed (t-test function in SpotfireDecisionSite, Functional Genomics module).
Asterisks indicate significant differences in relative expression (*P < 0.05; **P < 0.01).
Significant differences between female and male determined with t-test, asterisks indicate significant differences in relative expression (*P < 0.05; **P < 0.01), and NS means no significant differences.
The microarray data were validated by qPCR analysis of selected genes, although the fold in relative expression levels showed some differences for a few genes (Fig. 1G).
Detailed information such as fold change in relative expression of these transcripts upon gall midge infestation (Fold change RPI/RPUI), their gene locus ids, protein name and function, GO annotation, pathways, Affymetrix probe ids is provided.
Y-axis depicts the log2 values of fold change (FC) in relative expression values (REV) of genes (mentioned on X-axis) between infested (RP-I) and un-infested (RP-UI) samples.
Detailed information such as fold change in relative expression of these transcripts upon gall midge infestation (RPI/RPUI), their gene locus ids, protein name and function, GO annotation, Affymetrix probe ids is provided.
Nonetheless, at the present time, the reason for this difference in relative expression remains speculative.
Results demonstrated a marked increase in relative expression of Ncf1 but not Ncf2 or gp91phox in stimulated cells (Figure 6).
