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Limits of quantification were determined in relation to the method blanks and in relation to the quantification limit of the instrument, which is proportional to the sample size.
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The results obtained in the quantification of the wettability from the T2 relaxation times were very different in relation to the wettability quantification provided by Amott-Harvey Wettability Index.
Expression levels were calculated as absolute quantification in relation to the relevant standard curve, using Lightcycler Software version 4·05.
Quantifications were normalized in relation to the housekeeping gene rp49 [41].
Gene expression quantification was carried out in relation to the expression of the endogenous controls and the expression in the velvet following ΔΔCt method [112].
Finally, to overcome the uncertainty in relation to the complex visual investigation of dynamic loops at CEUS, quantification of CEUS is necessary, which may facilitate differential diagnosis between ICC and HCC [ 30, 31].
The quantification of these genera in distinct mangrove systems in relation to the local oil levels would permit the evaluation of the level of perturbance of mangroves.
The quantification of these genera in distinct mangrove systems in relation to the local oil levels would permit the evaluation of the level of perturbance of mangroves, being useful in field monitoring.
Divergence was calculated by comparing sequence pairs in relation to the phylogeny reconstructed by MegAlign.> Additional file 1: Gel quantification analysis of all bands by ImageJ.
In order to reduce the variation between different assays and samples, a procedure based on the relative quantification of target genes versus their controls in relation to the reference gene was used.
In order to reduce the variation between different assays and samples, a procedure based on the relative quantification of target genes vs their controls in relation to the reference gene was used.
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