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Post-hybridization washing was carried out in reduced lighting with 42°C 2X SSC (0.2% SDS) for 15 minutes followed by room temperature washes with 2X SSC and 0.2X SSC for 15 minutes each, then centrifuged until dry at 700 rpm.
Mice were returned to their home cage and placed in a different room than previously tested in reduced lighting conditions for a period of no less than 1 h.
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I've tried the camera in reduced light and it seemed to deliver more forgiving, sharper images than before.
The opacity of the metal framework in metal ceramic restorations results in reduced light transmission through the tooth that diminishes the esthetics of the tooth.
Closure of stomata results in reduced light-saturated stomatal conductance (g s sat ) and photosynthetic CO2 assimilation, (A sat ) affecting leaf growth and productivity (He et al. 2001).
Charles also recommended placing yourself in reduced light during a migraine attack, but cautioned that avoiding light between attacks could potentially make you more sensitive to the light and therefore more likely to experience a migraine when being in reduced light isn't an option.
In a greenhouse experiment diametric growth rate of foliar spots produced by D. mutila was higher in full sun conditions (mean 19.5±2.5 SE cm/day) than in reduced light (10.0±2.5 cm/day) and full shaded conditions (0.52±2.5 cm/day; analysis of variance (ANOVA), F3,30 = 12.62, P = 0.0001; Fig. 2).
Sample preparation was conducted in reduced light condition in order to minimize the pigment loss.
Some cognitive abilities decrease with age for example, the ability to distinguish facial patterns in reduced light (Hayes and Minardi 2005).
The taller and denser canopies (Meadows 2006; Mozdzer and Zieman 2010; Mozdzer and Megonigal 2012) and thick litter layer (Holdredge and Bertness 2011) in stands of the introduced lineage cumulatively result in reduced light availability.
Briefly, assays were performed in with reduced lighting, simply by the addition of 20 µl of CellTiter AQ reagent into the relevant samples in 100 µl of culture medium.
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