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T2D-like symptoms were induced in RCU mice using the insulin antagonist S961 as described above.
Figure 5A thus show live insulin-lineage tracing mCherry and Ucn3-GFP fluorescence in RCU islets cultured on 804G for two weeks.
Insulin expression in RCU progeny is permanently marked by red nuclear fluorescence, and Ucn3 expression is marked by green cytoplasmic fluorescence.
DOI: http://dx.doi.org/10.7554/eLife.02809.011 10.7554/eLiFigure09.012 Figure 5 figure supplement 1. Alk5 inhibitor II induces Ucn3 -GFP in RCU islets in a dose-dependent manner.
Shown is a dose-curve for the induction of Ucn3 -GFP in RCU islets de-differentiated on 804G matrix for 1 week, following by 1 week treatment with the indicated concentration of ALk5 inhibitor II.
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A dose response test showed that its effect on Ucn3-GFP expression in de-differentiated RCU β cells begins at nanomolar concentrations.
This concept views the soil resource as a dynamic 3-dimensional geomorphological landscape and presents information as regolith terrain (R T) data in the context of Regolith-Catenary Units (RCUs) in an explicit and repeatable process.
The experimental design, outlined in Figure 4A, employs healthy islets from adult RCU mice, isolated on day 1 and plated on an adherent 804G matrix in a 384-well plate format.
A list of all compounds and concentrations appears in Supplementary files 1. Fresh un-manipulated RCU islets were used as a positive control, and DMSO- or untreated islets were used as a negative control.
For the tasks implemented on the RCU, we assume that the hardware resources are sufficient to execute in parallel all hardware tasks chosen by the partitioning step.
(A ) Islets from adult RCU mice are isolated and plated on 804G matrix for 1 week in a 384-well plate format during which time the β cells de-differentiate.
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