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The pools were put on at 10 pM and run in rapid mode at 2 × 100 paired end.
Where necessary, additional sequencing (top off) was performed in Rapid mode sequencing (HiSeq2500) to ensure data analysis was completed in a clinically appropriate time frame.
The library with an insert size of ~470 bp was sequenced on a HiSeq 2500 v2 instrument in rapid mode as 2 × 265 bp reads, which produced 'stitched' reads of ~265 520 bp in length.
For this method, sampling of conformational space was done in rapid mode.
Focusing began at 250 V for 20 min in rapid mode, 10,000 V for 5 h in linear mode and 10,000 V for 50,000 Vh in rapid mode (for the IPG strips 5 8).
IEF for the strips 7 10 was carried out at 250 V for 60 min in rapid mode, 10,000 V for 3 h in linear mode and 10,000 V for 50,000 Vh in rapid mode.
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The reason why CAPs display activity against chemoresistant cancer cells probably lies in their rapid mode of action against the plasma membrane, resulting in lysis of the cells [ 17].
In our glioblastoma U251 model, the rapid mode of action of ASA404 became also apparent by the changes in tumor color 8 h post injection which indicates hemorrhagic necrosis.
If growth nucleus (small parts of microtubule) survives during de-synchrony, as shown in Figure 2b middle, then the rapid mode is triggered faster.
Purified libraries were size-selected for a range of 300 bp and sequenced on an Illumina HiSeq 2500 in the 101-bases paired-end rapid mode.
Each ChIP-seq library was sequenced with two complete lanes on the Illumina HiSeq 2500 in the 101-bases paired-end rapid mode and aligned to hg19 using bwa.
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