A 70-gene prognosis profile determined by microarray analysis appeared strongly predictive for outcome in young patients with breast cancer [ 6, 7], and is presently in the process of validation in other series of patients in different laboratories.
This instrument is widely used and validated for outcome assessment in a variety of general diseases, and is therefore often used in the process of validation of new scores.
In the process of validation, phenomena that affect the transient response of the cell significantly are identified.
Although the PKU-QOLQ is still in the process of validation for males and other age groups, it proved highly effective at assessing QOL in our study sample, when controlling for age, with no observed gender differences in scoring outcomes.
In the qPCR confirmation, based on the copy numbers of every individual predicted by RD and qPCR method, we systematically assessed performance of the RD-called CNVs through three evaluation criteria in the process of validation, including the overall agreement rate of RD with qPCR results, the prediction power of RD and the positive prediction rate of RD.
Participants will also complete the Visual Analogue Scale (VAS) of "urge to eat", "hunger", "tension" and "mood" and a short and modified version (comprising 5 items) of the Food Craving Questionnaire-State (FCQ-S) [ 59], which was translated to Brazilian Portuguese and is in the process of validation.
This study reports on the first step in a staged process of validation.
The use of different methods in a gradual process of validation with confirmation of the meaning of the analyzed data is recommended [ 35].
