Therefore, Fgf signaling has the same function in rosette formation and hair cell differentiation in postembryonic intercalary neuromast formation, as in primary neuromast formation during primordium migration.
The next phase is recruitment of mesenchymal cells that differentiate into osteoblasts resulting in primary bone formation with callus production followed by secondary bone formation, with bone remodeling.
Figures 3A, 3B, and Supplemental Figure S13 examine the role of POSTN in primary tumor formation and metastasis utilizing a MMTV-PyMT mouse model of mammary tumor formation.
In contrast to the biphasic pattern of the primary sphere formation in irradiated MCF-7 cells, we observed a dose-dependent increase in primary sphere formation in T47D cells, with a two-fold (P = 0.018) maximum increase at the highest dose of 8 × 2 Gy.
The defects in primary neurosphere formation in the presence of the Cdk4 R24C alleles actually occurred with independence of the levels of Cip/Kip inhibitors.
Lack of both p21Cip1 and p27Kip1 in a wild-type background resulted in a significant increase in primary neurosphere formation that was not present in the single knock-out cells.
We examined the highly conserved protein CCDC113 further and found that it is a component of centriolar satellites, is in a complex with the satellite proteins HAP1 and PCM1, and functions in primary cilium formation.
However, mtDNA did not seem to be involved in primary tumor formation: When the group swapped mtDNA from metastatic cells into normal cells, it did not cause these to form tumors.
Furthermore, collectrin is preferentially associated with primary cilium and is shown to play a role in primary cilium formation, cell polarity and maturation revealed by siRNA experiments.
In primary neurosphere formation assays, approximately 15% of control CD133+ cells formed neurospheres, whereas very few spheres were formed by cells depleted of c-Myc (Figure 6A).
