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To test this idea we carried out Illumina deep sequencing of ssRNAs from the rosette leaves of 4-week old wild-type Arabidopsis and the attex1 mutant of Arabidopsis (Elina et al, in preparation), in which ssRNA production from the TAS1 and TAS2 ta-siRNA loci, stimulated by mir173, is blocked.
This notion is supported by our recent data (Minskaia et al., manuscript in preparation) in which we show that the cleavage activity of F2As of 30aa (and longer) was insensitive to the C-terminal sequence of the upstream gene.
This application example makes use of a dataset from another study (in preparation) in which we investigated whether it is possible to distinguish between wheat samples of different years, farming schemes and cultivars.
We have also found a significant increase in CHD among cytomegalovirus-seropositive participants in the Newcastle 85+ study (Spyridopoulos et al, manuscript in preparation), in which we investigated 751 participants of the same age group (85 years).
As stated above, the analysis of this new series of conditional Dlx5/6 mutant is the subject of another paper in preparation in which we include also the use of other tissue-specific cre-recombinase mice.
"The authors then cite unpublished data for a CNCC-specific knockout of Dlx5/6 and state this has no phenotype in the maxillary arch, but fail to show this" The extensive analysis of this new series of conditional Dlx5/6 mutant is the subject of another paper in preparation in which we include also the use of other tissue-specific cre-recombinase mice.
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Further live-imaging analysis of interneuronal migration in mutant mice with radial glial cell deficits and in preparations in which the entire population of radial glia can be visualized, will be essential to fully establish the significance of interneuron-radial glia interactions in the modulation of interneuron migration dynamics.
In human microneurography, sympathetic maneuvers (e.g., valsalva) can be used to induce a response in some sympathetic fibers, but such procedures do not distinguish afferent and efferent fibers in preparations in which axons are cut and neuronal activity is recorded from the peripheral axonal process distal to the cut.
In contrast, in preparations in which the labelled probe was used for direct detection, the presence of background signal was detected in the cytoplasm of PMCs, impairing the detection of positive hybridization signals.
In 7-day-old flies the mean frequency of contractions was 5.7 ± 1.6/minute (in preparations in which the spermatheca and seminal receptacle and uterus had been removed to avoid mechanical coupling).
"Now, in a ritual reminiscent of the 1950s, when students ducked under desks and covered their heads in anticipation of nuclear blasts, many schools are preparing for, among other emergencies, bomb threats, hazardous material spills, shelter-in-place preparation (in which students would use schools as shelters if a dirty bomb's plume were to spread dangerously close) and armed, roaming sociopaths".
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