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C) Hydrophobic groups (yellow-dotted spheres) in position to interact with the lipid bilayer.
This created the first filter, to select for docked poses in the screening database with hydrogen bond acceptor atoms or negatively charged atoms in position to interact with the arginine residues.
The final filter simply selected for poses with a certain amount of hydrophobic bulk located in the region above the binding site, that would be in position to interact with the lipid membrane and with hydrophobic residues around the edge of the site.
Arg234 of IL-3R alpha is also in position to interact with Ser17 of IL-3 through hydrogen bonding.
The server requires a desirable starting position to place the protein interfaces residues in position to interact with each other.
When loop 5 is in the more open conformation, K88 is not in position to interact with the first purine nucleotide of the consensus, in this case Gua1.
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Although the detailed interactions at interface V of IL-3 await direct experimental determination, our modeling suggest that Leu32, Leu34 and Leu35 are in positions to interact with their counterparts in the symmetric partner, much like the leucine zipper interaction observed in many protein complexes.
Residues 305 314 on the surface of the first subunit are not in a position to interact directly with the binding pocket, but could be involved in mediating interactions between the two subunits.
Because our new structure makes it possible to visualize the side chains of IL-1α, it is now clear that Asp151 is in a position to interact only with IL-1RAcP, rather than the receptor, as observed for the cognate amino acid in IL-1β.
As seen in Figure 3, MR His-950, PR Arg-899, GR Lys-743 and AR His 885 are oriented away from the steroid binding site and are in a position to interact with other proteins.
Since only three acidic duplets of Apaf-1 are in a position to interact with cytochrome c (see Figs. 4 and 10 ), we believe that these acidic pairs might bind cytochrome c, thus triggering the apoptosome formation.
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