Although annealing usually can improve the crystallinity of the seeds, the peak spacing of the seed layers increases after annealing, resulting in poor alignment of the NWs.
However, the focus on interoperability resulted in poor alignment between local work processes and software specifications.
Where research capacity was slow to develop, this was reflected in poor alignment between the principles of RCB and the characteristics of the team, their activities or environment.
Current short-read aligners operating on a single reference genome are simply not designed to handle this scenario and will result in poor alignment accuracy.
3 A long-recognised risk factor for OA is direct joint injury, such as a subchondral fracture, meniscectomy, anterior cruciate tear or a deforming tibial or femoral fracture that results in poor alignment or leg length shortening.
- Multiple, shifting and conflicting objectives that lead to difficulties in predicting how incentives will align and how they will be reacted to; - Weak institutional and governance environments on both sides of the aid contract that result in poor alignment of activity within relevant organizations as well as between them.
It is within these repetitive regions where most indels and amino acid differences seem to accumulate, in some cases resulting in poor alignment, and therefore they could be responsible for the surprisingly high amino acid and indel evolution of Hox and Hox-derived proteins.
When alignments of O. tauri proteins contained gaps, extended inserts which were not found in other species, or poor alignments, we investigated and where appropriate corrected underlying gene models.
In order to exclude poor alignments and reduce ambiguity, we filter out alignments where less than 85% of the RNA is aligned, or the sequence identity is less than 90%.
As we previously published, to filter out potentially spurious SNV calls in these regions, mutant samples were compared to the relevant parent line to remove areas that had poor alignments in either sample [ 7].
