If we consider the general trend of concentration of the analytical process in large laboratories, the design and planning of sample collection routes to minimise travel time becomes especially relevant.
Without denying the existence of protocols that do produce highly similar results, we would like to emphasize that the significant differences observed between the two established methods point out the need for a careful planning of sample preparation in RNA-Seq experiments.
JG participated in the planning of the study, acquisition of samples, culture and isolation of mycobacteria and molecular analysis of isolates; BBA participated in planning of the study, training JG, supervision of the molecular assays, data analysis and drafting of the manuscript.
Further research has shown the extent of the lack of use of systematic reviews in planning the targeted sample size calculations [ 6] as well as the design of the interventions [ 7].
KKL participated in planning, sampling and evaluation of the manuscript.
MCID will also be useful in the planning of new trials, as sample size depends on the magnitude of the difference investigators consider clinically important and are not willing to risk failing to detect [ 34].
It will also be useful in the planning of new trials, as sample size depends on the magnitude of the difference investigators consider important and are not willing to risk failing to detect.
LG participated in the planning of the study, the sampling of the fish, carried out the molecular biology, participated in the statistical analysis, in the meta-analysis and drafted the manuscript.
PC participated in the planning of the study and in samples collection from patients.
ROL participated in the planning of the screening campaign and sample collection.
