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The CLUSTAL X gene alignments were checked by eye to obtain correct outputs in PHYLIP format.
Submitted nucleic acid sequences or protein sequences in FASTA format are computed for the output alignment in PHYLIP format using ClustalW; a query initiated in a pre-aligned file in PHYLIP format by other sequence align tools disregards this procedure.
The input for PALM can be either sequences in FASTA format or a sequence alignment file in PHYLIP format.
The amino acid replacement and conservation patterns were studied where two aligned amino acid sequences in PHYLIP format were compared and the amino acid replacements were quantified.
Meanwhile, pre-aligned sequences in PHYLIP format can be accepted by PALM as input to fulfill user requests with respect to alignment from various perspectives.
Alignment files were converted in phylip format.
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In order to be processed by ProtTest and Phyml, the alignments were converted into Phylip format.
These high-quality SNPs were extracted and converted into PHYLIP format multiple sequence alignment for phylogenetic analysis with RAXML v8.2.848 under a GTRGAMMA model (-m GTRGAMMA).
Multiple FastA was converted into PHYLIP format (for tree building) with the EMBOSS program Seqret (25 ).
The amino acid sequences were aligned by the algorithm Prank (Löytynoja and Goldman 2005) and transferred it into phylip format by trimAl (Capella-Gutiérrez et al. 2009).
(iii) Input the tree file in the Phylip format.
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