Cell lysates were used in two parallel procedures: (1) GTP-Ras levels were determined by the glutathione S-transferase-Ras-binding-domain of Raf (RBD) pull-down assay as previously described [ 46], followed by determination of activated Ras levels by pan-anti-Ras antibodies (Cat. # OP40; Calbiochem, Gibbstown, NJ, USA) using WB.
In case of healthy control animal (HK31), endoscopy procedure was also performed twice in parallel with procedures done in gluten-sensitive animals despite that no relapse stage characterized by significant antibody increase was present in HK31 in association with introduction of dietary gluten (Figure 1).
In a parallel procedure, the pSF-04 expression vector was digested with the Mfel restriction enzyme to excise the insert bearing the lacZ encoding sequence.
In a parallel procedure, fat body was directly isolated from each group of pupae (n = 10).
In general, the parallel procedure consists of the following two steps: 1. Subgraph retrieval For every vertex v in the graph G, retrieve the induced graph (G_v) consisting of v and its neighboring vertices in G; 2.
With this tool in hand, we construct a parallel procedure for the localization and isolation of all the roots by dividing the given region successively and applying the above formula to these subregions until the final domains contain at the most one root.
(B ) A parallel procedure is carried out for 3000 surrogate datasets, in which responses are randomly exchanged among the stimuli.
The entry procedures are outlined below: Parallel Procedure.
Sections from mature and old monkeys were always processed in parallel following identical procedures (e.g., 3,3′-diaminobenzadine incubation time).
To control for technical variation, control and transgenic embryos were processed in parallel, using identical procedures and reagents in a 24-well plate.
