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In parallel preparations, Alexa Fluor® 488 hydroxylamine, mouse IgG and mouse and human IgG F ab' 2 fragments were reacted with aldehyde SPIO at room temperature in PBS buffer to obtain control reagents for Fc blocking studies and to synthesize combined fluorescence and antibody conjugated SPIO preparations.
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In parallel, PBMC preparations were seeded onto fibronectin-coated eight-well chamber slides (2 × 10 cells per well) and cultured as above.
In parallel, microsomes prepared from yeast expressing CYP98A3 were perfectly functional and consistent with the results described elsewhere, which showed that the preparation of microsomes was efficient.
The following execution trace will be expected from the agent in this case: External stimuli s 1, s 2 and contexts c 1, c 2 will occur in parallel and trigger preparation of actions a 1 and a 2. Based on the preparation states for a 1 and a 2, the sensory representations of predicted effect b 1 of a 1 and b 2 of a 2 are generated.
Administration of the projects In parallel with military preparation in the lines of fighting and the camps, a committee is to be put in place to administer production projects and put in place plans to implement new investment projects.
Some ECHA guidance documents were elaborated in parallel to dossier preparation; others have been amended already several times.
In parallel with the preparation of the testbed, soil samples must be collected and sent to a specialized laboratory for soil analysis.
Each cECG measurement lasted up to 30 s and was performed in parallel to the preparation in the catheter lab shortly before starting the acute coronary angiography.
In parallel to probe preparation, cells used for FISH experiments were fixed in 4 % paraformaldehyde in PBS (EM grade, Electron Microscopy Science, USA) for 10 min at 4 °C.
Control experiments indicated that the (His 6-tag did not stimulate overall recombinant enzyme activity, since tHis 6-tag rF6Hb treatedidnotarallel with the preparation undergoing cleavage exhibited a stimulateeductioverallnzyme activity.
We dissected and processed the cortices or cerebellum of individual mice separately and in parallel, because immediate preparation prior to PCR genotyping was necessary for obtaining optimal neuronal cultures.
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