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Similar is the case of the salicylic pathway where only the transcription factor TGA is found upregulated in our transcripts.
The annotation results, however, did not pick up oxalate oxidase or oxaloacetate acetylhydrolase (enzyme involved in conversion of oxaloacetate to oxalate) in our transcripts.
In our transcripts, contact events triggered efforts to recall—'did I actually perform HH first?' Memory of an HH event can depend on the ease of retrieval of the last sensorimotor experience of hand washing, which leads to the recognition that an error was made.
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We used an iterative mapping approach to identify and define de novo splice sites with short reads and increase confidence in our transcript predictions.
Ten genes known to be imprinted in placenta had sufficient expression levels to attain a read depth that provided statistical power to detect imprinting, and yet all were consistent with non-imprinting in our transcript count data for neonatal brain.
However, another mechanism may be suggested in our transcript profiling data, which illustrates a reduction in transcripts associated with terpenoid biosynthesis, which lies upstream of ergosterol production.
No such correlation could be detected in our transcript profiles from many tissues of Setaria (Additional file 1: Figure S2).
While most of the JGI set was found in our transcript collection, we had many transcripts not covered at all by the JGI set (37%).
Thus, it is not surprising that one of the most affected metabolic pathways in our transcript profiling experiments in this study was steroid biosynthesis.
Gene expression programs associated with activation or proliferation of specific cell types were also evident in our transcript data and Gene Ontologies.
ABA is another notable terpenoid observed in our transcript annotations which has anti-diabetic, anti-inflammatory, anti-obesity and immuno-modulatory properties.
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