Exact(1)
Then, we used these percentages in our permutations, but noted the total expression level of each gene in each library.
Similar(59)
To model this assumption in our permutation test, in each round, we permuted tissue labels for each PAS cluster; then, for each gene, the percentages of the permuted PAS were used to represent a multinomial distribution, from which we drew a random sample, scaled by the total gene expression value in each tissue.
In our permutation test, this accuracy was statistically significant (P < 0.048).
Hence, we applied several criteria with a lower stringency in our permutation method, such as a percentage of probes (not necessarily continuous) above the cutoff within a given window, and we combined peaks that were ≤2 kb apart.
That means most pseudo pathways in our permutation design met the requirement of gene expression correlation, although we did not limit the correlation values of genes when generating pseudo pathways.
At the end of S (5000 in our case) permutations calculate p value as, p = C S. The p-value obtained after step 7 represent the probability of co-occurrence of two CNAs in absence of any other CNA in sample.
Regardless of the cause, we believe we have accounted for the effects of this skew in our analysis; permutation of the data preserves the ancestry skew at each site and in coalescent simulations of hybrid populations we explicitly modeled skewed admixture proportions to match the data.
Besides its use in statistical tests, our permutation procedure for generating surrogate synchronization data helps to ensure that our measures of synchronization are independent of the effects of volume conduction.
In accordance with our permutation test, logistic regression analysis revealed that NAF patients pertaining to the HV* haplogroup aggregate had a threefold risk of developing nephropathy [p = 0.027, OR = 3.12 (1.14–8.6)] and a fourfold risk of developing cardiovascular disease [p = 0.014, OR = 4.13 (1.33 12.9)] (see Additional file 1 – Table 4).
Therefore, in our analysis, both permutations keeping the first order nucleotide frequencies and permutations keeping the second order nucleotide frequencies were used.
For example, if all derived SNPs were found on serpentine, then granitic populations would have higher average intensities in significant regions; because our permutation samples only from significant regions, the candidate CNV are significantly beyond any such effect.
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