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A central point in our assignment procedure is to assign a weight w(p, q) to matching segment sets p ⊆ P onto q ⊆ Q.
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We further used two outside coders to assess our coding scheme independently to increase confidence in our assignment of codes to appropriate categories (Gioia et al. 2013).
We were confident in our assignment of the break-point between the 5' and 3' sections because CR1 and CR2 sequences were either identical or differed by a maximum of two mutations downstream of the breakpoint.
Interestingly, the Cbs homologs identified in nominally aposymbiotic samples (A. elegantissima and F. scutaria) showed greater sequence similarity with Symbiodinium gene models than coral (A. digitifera) and were classified as dinoflagellate in our assignment procedure, whereas Cbs homologs in symbiotic samples (M. cavernosa and S. hystrix) included both metazoan and dinoflagellate transcripts.
In our assignment procedure, we will refer to the four host races that have been unambiguously identified (Cucurbitaceae, cotton, Solanum and pepper) from numerous samples of A. gossypii collected over several years on a large geographical scale from annual crops of different plant families [ 22].
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Although we combined information from several databases to infer protein functional locations, uncertainty still exists in our assignments.
In our assignments, students learned the process of constructing phylogenetic trees through a series of increasingly difficult tasks; thus, skill development served as a framework for building content knowledge.
We were conservative in our assignments and only further considered offspring whose father was known with greater than 95% confidence – 44 out of 94 offspring in the LP1 versus HP trial and 16 out of 32 in the LP2 versus HP trial.
Such correlations or associations can be powerful aids in enhancing our assignment capabilities in these complex cell wall samples.
Hence, crosslinks to these positions would be expected in case our assignment of the two electron densities would be inverted.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com