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Three more Amsterdam cruises out of Port Everglades brought down another 261 passengers, and the shipping company decided to cancel a cruise in order to swab and steam clean the Amsterdam.
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In order to avoid swab sample variation that can occur when multiple swabs are taken, the clinical samples were divided into two groups (I & II).
Further testing of this method in controlled trials is warranted in order to determine whether oral swab PCR can play a role in widespread screening and diagnosis for MTBC infection in the primate biomedical model.
During screening subjects had the chance to chew on a swab in order to get used to the procedure.
In order to minimise contamination, each nasal swab was incubated at room temperature in distilled water containing 3000 IU/mL benzyl penicillin for 3 h prior to plating.
Five hundred microliters of the suspensions were spread over the plates containing Mueller-Hinton agar using a sterile cotton swab in order to get a uniform microbial growth on both control and test plates.
Afterwards, the inner side of the insert was wiped with a wet swab, in order to remove the cells and the outer side of the insert gently rinsed with PBS and stained with 0.25% crystal violet (Sigma) for 15 min, rinsed again, and then allowed to dry.
Therefore, in order to assess the influence of time from swabbing to extraction on DNA yield and purity, buccal swab samples from the same person (n = 4) were collected and stored for 2 weeks, for 1 week, for 1 day and for a few hours in the collection medium (buffer and enzyme) before DNA was isolated.
This has led some authors to conclude that S. aureus screening should include both nose and throat swabs in order to allow a complete evaluation [ 12].
In order to avoid bacterial growth, buccal swabs, sent to the laboratory by mail, were packed into screw cap tubes with ethanol.
In order to quantitate the bioburden of the swabs, pour plates were performed to obtain the colony count (CFU/ml).
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