Two-dimensional UTE sequences [19] require twice the number of RF pulses in order to achieve slice selection, which doubles the acquisition time.
In order to handle slice selectivity, i.e. the location of the image section plane [3], standard MRI excitation schemes are used [3, 59].
Spines were inserted at angles to each other (typically ~30°) in order to facilitate slice identification (see below). Figure 2a shows the appearance of a spine on a haematoxylin and eosin (H&E -stained H&E -stainedgy slice at a spatial resolution of 1 μm/pixel.
Kynurenic acid and indomethacin were included in the cutting medium in order to improve slice viability [17].
In order to load slice cultures prepared from wild-type mice with CoroNa Green AM (Invitrogen, Carlsbad, California, USA), 5 µL 20% Pluronic F-127 in DMSO (Invitrogen) was added to a 50 µg vial of CoroNa Green AM.
We therefore chose to perform whole-body image acquisition with respiratory gating in order to minimize slice position mismatch between different b values and different excitations.
For tissue slicing in the microtome, samples were properly positioned in order to obtain slices with the same orientation with respect to the optical sectioning performed with two-photon microscopy.
However, in order to apply slice-level parallelism to a HEVC encoder, we need to focus on CTU structures, variable block sizes, and coding modes.
It will be used to virtualize the transport network in order to support slicing and to allow a flexible deployment of virtual functions in different places of the network, as is required for the support of flexible functional splits.
PET VOIs were 8.1-mm thick (4 PET slices) in the axial dimension in order to match the slice thickness of the MR data (8 mm).
