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The study overcomes limitations in prior studies in that it (1) assessed personal characteristics before study entry in order to exclude "contamination" effects, (2) selected an appropriate control group, and (3) differentiated between STEM and non-STEM study majors.
In order to exclude contamination and check for likely functionality, we analyzed genomic features and EST (expressed sequence tag) data.
All bark samples were washed with double-distilled water in order to exclude contamination of phloem exudates with xylem sap.
In order to exclude contamination error as the mechanism for superinfection events, multiple time points were assessed and amplification of NS5B was carried out at different time points.
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In order to exclude contaminations with mouse genomic DNA, MLV positive samples were also assessed by a nested PCR targeting mouse histone deacetylase 5 (Hdand) andisplayingng the same sensitivity as the nested PCR used to detect MLV.
Two negative controls were included for every 8 samples tested in order to exclude contaminations during the nested reaction as well as a positive control for 16 reactions.
We also examined the pixel level data of flare stars in order to exclude the contamination of flares from nearby stars or other false events.
As T cells do express CD3γ and CD3δ mRNA, these lanes were used as a control in order to exclude a contamination by ordinary T cells.
In order to exclude the contamination of PLF with cells of the monocyte/macrophage lineage, fourth passage PLFs were characterized by flow cytometry.
A no template control (NTC) reaction was included in every run for each primer pair in order to exclude DNA contamination in buffers/solutions and to assess primer dimers.
We, however, employed RA FLSs between passages 4 and 10 in order to exclude a contamination of other cell types because approximately 30% of the synovium is composed of macrophage-like synoviocytes.
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