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In order to determine responsive sRNAs for alternate bearing, six small RNA libraries were constructed from "on-year" and "off year" leaves in July (JON and JOFF, respectively), again in "on-year" and "off year" leaves in November (NON and NOFF, respectively) as well as with ripe (RF) and unripe (UF) fruits.
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In order to determine whether spt mutants are responsive to reduced levels of GA, we exposed seedlings to the GA biosynthesis inhibitor paclobutrozol (PAC) and examined the effect on meristem size.
In order to determine whether our top hypoxia responsive miRNAs (miR-185-3p; miR-485-5p; miR-216andp miR-625-5p miR-625-5p miR-625-5ptheir putative promoter region we scontain 600 bp flanking sequences upstream of tHREsranscrintheirstart site of the putativemiRNA.
In order to determine how data on nitrate-responsive genes obtained with RNA-seq and Affymetrix ATH1 chips correlated, we calculated the correlation between the KNO3/KCl ratio for RMA normalized Affymetrix gene expression and the KNO3/KCl ratio obtained for normalized libraries at different average gene coverages (AGCs).
In order to determine whether there might be PR-responsive elements in the Stat6 promoter or other regulatory elements we have conducted luciferase assays on the cells cotransfected with vectors carrying PRB cDNA and Stat6 promoter sequences and none significant changes have been detect.
In order to determine if miR5640/AtPPC3 could represent a nitrate-responsive miRNA/TARGET module, we analyzed the nitrate response of the miR5640/AtPPC3 pair on a time course using RT-qPCR.
In order to determine the transcriptional outcome of p53 binding to NGF-responsive target regions described above, RT-PCR was performed to compare mRNA levels for indicated targets among naïve and differentiated PC12 cells.
Data were then extracted from each individual retinotopic ROI for the main experiment, for statistical analyses outside SPM, now considering left and right target trials separately (for right or left target-responsive retinotopic ROIs, respectively), in order to determine any TMS and/or validity effects in these ROIs responding to contralateral targets.
In order to determine the pattern of mutation and polymorphism across putative HC responsive regulatory elements in SCD patients, we designed sequence-specific primers corresponding to a 2·3 kb fragment of SAR1A on chromosome 10 which was completely sequenced in all subjects.
The expression of salt-responsive genes was analyzed for each sample in order to determine the suitable time point for samples to be sent for sequencing (Additional file 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2014/467395).org/10.1155/2014/467395
In the present study, a cDNA microarray approach was taken in order to determine if the transcription of genes, from a set of previously identified putative stress-responsive genes from chickpea and its close relative Lathyrus sativus, were altered in chickpea by the three abiotic stresses; drought, cold and high-salinity.
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