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All the tasks were carry out in end expiratory, open glottis apnea in order to avoid variation in autonomic activity due to breathing.
In order to avoid variation, all samples were studied on the same day and using the same kit.
In order to avoid variation of size, depth, and location of myocardial injury, we used a cardiac cryoinjury model as previously reported [ 12].
In order to avoid variation resulting from differences in the amount of immunoprecipitation protein between samples, the phosphatase activities were normalized to the amounts of PP2A immunoprecipitated, which were detected and quantified by immunoblot analysis for each treatment group.
For at least 7 days prior to the test, we separated the water striders according to their sex (30 individuals per 30 × 40 cm container filled with water) in order to maintain a similar level of sperm storage in males before the experiment and in order to avoid variation among females in the amount of sperm already received through recent copulations.
The evergreen forests of Andaman Islands are generally of low elevation and gentle slope [ 34], and in order to avoid variation in estimates due to different site characteristics, we restricted sampling to elevations between 0 and 275 m asl with mean elevation of all quadrats being 74 ± 5 m.
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In our study, the IWI was measured with a fixed FiO2 of 35% in order to avoid variations in SaO2 due to FiO2 variations.
It is clear that levels of biomarkers will need to be compared between equally treated samples in order to avoid variations in noise from the different ways the samples were prepared.
The relative force index (N/kg), calculated by dividing the total power by the speed, in order to avoid variations due to different speeds, and/or to different types of gait.
We decided to choose a retest interval of 2 days, similarly to the authors of the Spanish or Iranian version of NDI, in order to avoid variations in the clinical status of the patients and to avoid the patients remembering their previous answers.
As a sampling strategy; the samples were acid digested in triplicate in order to avoid variations caused by potential inhomogeneity causing fluctuations in elemental content.> -wrap-foot> For the evaluation, if WEEE was used for the production of black FCAs, Sb was presumed to be a key element.
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