Due to the broad emission spectrum of Thio-S we used only Cy 5-coupled secondary antibodies in order to avoid any possible overlap of emission spectra.
This is necessary in order to avoid any double sampling because of the 60percentt overlap if every photograph is sampled.
In the inset of Figure 5B, however, we calculated the female response time from the end of the male playback bout, in order to avoid any issue introduced by our difficulty in scoring overlaps in this particular experiment.
Hence, an internal feedback loop in the Ministry was necessary in order to avoid thematic overlap with the currently funded research areas and to achieve as high political relevance as possible.
In order to avoid the overlap of green and red fluorescence in a dual heterozygous assay, modifications were made to the previously described assay.
The emission was detected with three photomultiplier tubes in the range 425 475, 510 550 and 575 635 nm for the three lasers, respectively (in order to avoid channel overlap).
In order to avoid this overlap in the Fourier domain, we additionally conducted the cross-frequency analyses by using short Morlets ( f/ σ f = 12).
Viral proteins were excluded from these data sets in order to avoid the overlap among protein domains shared between viral and retrotransposon-encoded proteins resulting in final data sets of 39,252 PDB and 20,732 Swiss-Prot entries.
This is in direct contrast to multicolor FCM, in which careful consideration of fluorophores must be taken into account in order to avoid spectral emission overlap, and in which the use of highly complex analysis is necessary when overlap does occur.
"Our process tries to identify the specialists who have very defined areas of expertise in order to avoid overlap," Girish Reddy, a founder and managing partner, said.
