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However, severe hypoxemia (PaO2 <40 mm Hg) never occurred and PaCO2 was kept in a normal range in order to avoid alterations in renal blood flow due to changes in gas exchange [ 11].
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Try to always be respectful to both locals and other travelers in order to avoid altercations.
After PM and NPs exposure, before TEER measurements, transwell filters were washed with PBS and incubated with M199 medium for 10 min in order to avoid alteration in TEER values due to cellular debris and to insert handling.
In order to avoid such alterations in cell state, we have designed a procedure for the creation of B cell lines directly from murine bone marrow.
Special care is devoted to the embedding process of the optical fibre sensor in the composite plate in order to avoid significant alteration of the optical power spectrum of the light reflected back by the fibre Bragg grating.
Polyurethane foam was selected as the bonding agent, between the wooden framework and the soil block in order to avoid any possible alterations of the soil.
In order to avoid transcript abundance alteration in the sample, the minimal number of 17 amplification cycles was used.
We purposely chose a relatively short time point (8 h), in order to avoid a potential alteration of results by the differing cellular growth rates.
Another hypothesis is that urothelial cells with mitotic failure are exfoliated at a higher rate as a clearance mechanism in order to avoid the accumulation of genomic alterations in the target tissue [ 9].
Another exception allows for the alteration of work in order to avoid committing a criminal offence, such as one of those under the Obscene Publications Act 1959.
Thus, in order to avoid false/erroneous classification of miRNAs as up- and down- regulated in mucosal biopsies of IBD patients, only miRNAs with alterations of expression that fitted stringent thresholds (2−ΔΔCT>5-fold and 2−ΔΔCT<0.2-fold, respectively) were considered.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com