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To develop a random small RNA expression library suitable for large-scale genetic selections, we constructed a modified pLXSN retroviral vector containing the human U6 and H1 RNA polymerase III promoters in opposite orientation to each other (designated pHybrid).
The gene is located within 1.5 Kbp of the 3' end of and in opposite orientation to SECP43.
incA is located within the small intergenic region between repB and repC in opposite orientation to these coding regions.
It was demonstrated that the nuclear chl- fus gene locates in opposite orientation to a hop gene in Glycine max.
Integration site analysis showed that the provirus was integrated within the intragenic region, in opposite orientation to the SMC5 gene.
SuhB was first discovered in A. tumefaciens in opposite orientation to the suhB gene encoding an inositol-monophosphatase [ 35].
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The monomers are arranged with their N- and C-termini in opposite orientations to form an "open" conformation.
Interestingly, those genes that mapped to many piRNA sequences were nearly all oriented in the opposite orientation to the majority of piRNAs in the cluster.
We also determined that the CEN6/ ARSH4 cassette in pRS313 and pRS413 was inserted in the opposite orientation to those in other pRS CEN plasmids.
The promoter and proximal enhancer in clone pDEH7 were in the opposite orientation to the gene, and this region (-1895/+106) was deleted in clone pDE2.
The 3.5 kb fezf2 promoter was placed in the p2 site in the opposite orientation to the p1 NβT-tauGFP cassette to minimise potential promoter interference.
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