For the statistical tests, individual gene variances have been moderated using an Empirical Bayes approach as described in Siddique et al. [ 17] and in the online methods (Additional file 5).
Leg discs and salivary glands were stained as described in the online methods.
Animal breath metabolome patterns were analyzed as described in the "Online Methods".
The assay setup and compound isolation procedures are described in the online Methods section.
An efficient method for computation of GR using a public domain statistical package R does not require storing R and is outlined in the online methods with the R [ 13] script provided.
Tissue culture media, supplements, and reagents were as described in detail in Supplementary material online, Methods.
Oxidative burst in pooled RPM and SVF macrophages was measured using Fc OxyBURST Green assay (Molecular Probes; described in detail in Supplementary material online, Methods).
In situ hybridisation (ISH) was carried out using the methods described in online supplementary methods.
Immunohistochemistry (IHC) was carried out using methods described in online supplementary methods.
