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For radiation treatment, mice were immobilized in a customized harness facilitating exposure of the left flank, whereas the remainder of the body was shielded by lead. 4 Gy radiation was delivered locally in one fraction on day 7 using an MBR-1505R2 X-ray generator (Hitachi Medical Co).
The NMA allo SCT transplant regimen was administered in the outpatient setting using a conditioning regimen of oral fludarabine 42 mg/m on days −4 to −2 followed by 2 Gy of total body irradiation (TBI) on day 0 in one fraction.
The NCCN guidelines recommend that 800 cGy in one fraction be utilized over a more fractionated regimen, such as 3,000 cGy in ten fractions, for nonvertebral metastases.
The TCPs for absorbed doses of 60 Gy in a 300-mg tumour are 77% for 90Y, 74% for 111In, and 48% for 177Lu when given in one fraction, according to Equation 4. With fractionated therapy, the TCP drops to 21%90Y0Y), 16% (111In) and 12% (177Lu), despite the same dose of 60 Gy.
However, despite these promising results with fractionation protocols, a later study by Kolby et al. demonstrated that the SSTR2 receptors were saturated when doses above 30 MBq were given in one fraction [27].
Here, we have chosen to extract total RNAs in one fraction.
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Other authors [ 46, 47] preferred to fractionate, by means of sieves or pneumatically, the whole seeds into one fraction enriched in protein and oil and another one, with a great concentration of starch.
In this approach, one fraction of vesicles is labeled with two fluorescent molecules, with one of these molecules quenching the fluorescence of the other.
For 39 genes we found a decreased mRNA level at pH 9 in comparison to pH 7.5 whereby for 26 corresponding proteins a lower content was indicated by lower peptide numbers in at least one fraction and for four of them a significant lower abundance at pH 9 was observed (Table 1).
It has been earlier suggested (Rutherford and Harris 1940) that native sericin is in fact a homogeneous protein, and the existence of more than one fraction in the product of the extraction procedure is an artifact caused by degradation.
In one study, increased anxiety was observed in patients treated with one fraction, compared to those administered 10 fractions (Gaze et al, 2001).
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