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We therefore extracted genes that were differentially expressed from time points 5 to 6 specifically in one differentiation pathway.
As a control, neuronal cells were derived from the female hESC line H9 in one differentiation experiment.
For in-vitro differentiation, embryoid bodies (EBs) were generated from AD-iPSCs in one differentiation experiment each by cell harvesting and seeding onto low-attachment dishes in DMEM supplemented with 10% FCS, nonessential amino acids, L-glutamine, penicillin/streptomycin and sodium pyruvate (all from Invitrogen) without bFGF supplementation.
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The expression in different brain regions of the Alzheimer-exclusive genes that were found to be down-regulated in AD-iPSC neurons of one differentiation experiment were investigated using the GNF/Atlas organism part.
One differentiation agent used in the clinic is retinoid acid (RA) (vitamin A) [ 68].
In one example, adaptive differentiation of native insects flushing on invasive plants arose from rapid epistatic divergence of novel, major genetic effects (Carroll et al. 2003).
In one experiment the differentiation was continued until day 30 to induce an osteocyte-like state of terminal differentiation.
By comparing 383 GRG (Additional file 4) expression in MSC differentiated in one or the other differentiation pathway we retained 67 genes specifically associated with the myogenesis out of 383 studied.
Mutation in Cdc27 caused modest decrease in growth and differentiation, and mus304 prompted moderate overgrowth and high incidence of lamellocyte differentiation in one or more lobes.
The neural progenitors and neurons were paired if they were differentiated from the same iPSC clone and plated for differentiation in one experiment.
In their analyses of SUV in TC and higher grade LNET, Kayani et al. [19] categorized SCLC and NSCLC with NET differentiation in one group and LCNEC together with AC into another group of NEN which is not in accordance with the WHO classification [4] and is also distinct from the classification suggested by Rindi et al. [14].
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