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The hard part is in "normalizing" to create a single "service item" that can be scheduled, paid for, and "delivered" with a mouse click or smartphone tap.
RT-quantitative PCR amplification was performed using an ABI Prism 7900HT instrument (Life Technologies).Relative gene expression levels were calculated by a comparative Ct method that results in normalizing to PPIA(PeptidylprolylIsomerase A (Cyclophilin A))expression for each sample.
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In contrast, normalizing to gene copy number may be simpler and more reliable.
Independent experiments were performed in triplicate and average fold change in expression normalized to expression in undifferentiated GRP cells grown in bFGF.
The ANGPTL3 IHC scores of patients with primary T3/T4 OSCC tumors in primary OSCCs normalized to those in normal tissues.
Arteriolar vasorelaxation responses to Ach were expressed as the % change in diameter normalized to the baseline.
Results are shown as fold increase in luciferase normalized to the Renilla activity.
(C Nucleararimim fluorescence intensities of nuclei in (B ) normalized to −Ran condition.
The high quality reads were in silico normalized to reduce sequencing coverage.
miR-21 expression in media, normalized to cellular proliferation did not significantly influence these relationships (supplementary Fig. 3).
An example of variation in cell length normalized to its mean length is given in Figure 4 c.
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