We classified exons in NMD candidates as NMD-specific exons (the exons observed only in NMD transcripts) and NMD-nonspecific exons (the left exons in NMD candidate genes) (Table 3).
However, it is unclear how many genes that are misregulated in NMD mutants are directly targeted by the NMD pathway vs. those genes that are indirectly targeted, and which direct NMD targets are responsible for the organismal defects observed in NMD mutants.
It promotes a series of molecular transitions that are a hallmark of conversion from the PTC recognition complex to the mRNA degradation complex resulting in NMD activation.
Thus, DHX34 promotes mRNP remodeling and triggers the conversion from the SURF complex to the DECID complex resulting in NMD activation.
NCBP2 forms a heterodimer with CAP80 and plays a key role in the biogenesis of mRNAs, snRNAs, and microRNAs, and also in NMD.
In addition, microarray expression studies have clearly indicated that mRNAs with no obvious NMD features are also stabilized in NMD mutants (Rehwinkel et al, 2005).
a the NMD-specific exons are only observed in NMD transcripts, and the left exons of NMD candidate genes are classified as NMD-nonspecific.
We assessed its potential role in NMD in Drosophila embryos by means of a previously described NMD fluorescent GFP reporter [ 22, 23].
In addition, strains lacking the Ebs1 protein, which is homologous to the metazoan NMD factor Smg7 and contains a 14-3-3 14-3-3 14-3-3ndomainhosphoproteins, show partial deforts in NMD (Luke et al. 2007).
To measure DUX4-induced changes in NMD efficiency, cells were infected with lentiviral DUX4 or GFP 24 hr after transfection of the NMD reporters.
Our results showing a feedback loop between NMD and the UPR suggest that either the variability in UPR activation results from variability in NMD efficiency or vice versa.
