Pair (7, 9) is located in the loop connecting strand d from one repeat to strand a in the next repeat for and pair (25, 27) in a β-turn linking strand b and c within a repeat (fig. 4a).
Given the physical distances involved and the expected average size of DNA in solution, this prompted investigation of whether the minor product represented fusion of the centromeric flanking amplicon to the copy of DEFA1 in the next repeat (repeat 1).
Having established, in a sample of known haplotype composition, that mixed positions in the allele-specific sequence data were due to fusion of the flanking amplicon to the gene in the next repeat on the same chromosome, the sequence data for both 5-copy individuals was examined at the 8 variant positions within the gene.
The next repeat in row has been observed in 19.5% of cases (343 clones) and belongs to the highly abundant satellite family pEV [EMBL: Z22848] that forms large arrays in intercalary heterochromatin of each chromosome arm [ 32].
For example, we find that 28% of the mutated repeats are within 3 bp of the next repeat in the genome and 51% are 7 bp from the most adjacent repeat.
Although no single 100 bp read will cover a whole satellite monomer, if satellites are arranged in head-to-tail tandem arrays, then a predictable proportion of reads should cover the tail of one repeat unit followed by the head of the next repeat in the array.
The GAGAG hotspot for MM is located at the 3' end of each repeat, such that MM causes an almost full-length ubiquitin moiety to be fused with part the next repeat in the +1 frame, thereby creating an altered ubiquitin protein with a C-terminal extension of 20 amino acids, called hUbb+1.
join to the 9th d.c.; * 7 chain, miss 2, 1 d.c. in next, * repeat 9 times the 10 and 11, join to the other side and fasten off.
Join thread at corner; * ch 2, sk 2 ch, dc in next ch; repeat from * across.
With cotton No. 14 and hook 3, ** 1 s.c. on 5th d.c. of 1st division of h, * 5 chain, 1 s.c. in next division, repeat * 5 times more, 9 chain join to next point of g "9 chain," 1 s.c. in next point of g, 9 chain, repeat ** from all round; d.c.
join to the same place, where you began the long chain of f 5 d.c., * 5 chain join to 8th d.c. of f from where you joined the d.c.: 6 chain, turn, 1 d.c., 7 long, 1 d.c., miss 1, 1 s.c. in next d.c., repeat * 4 times more.
